[General control of amino acid biosynthesis in Hansenula henricii]

The general control of amino acid biosynthesis was investigated in Hansenula henricii. By limitation for single amino acids in wild type strain and mutants no derepression of enzymes was caused. In prototrophic revertants however, obtained from auxotrophic mutants (his, pdx) enzyme activities were 2 - 17 times higher than in the wild type. In these revertants enzymes of the biosynthetic pathways for tryptophan, phenylalanine, tyrosine, arginine, histidine, cysteine, methionine, asparagine, threonine, and isoleucine were derepressed. The amino acid pool pattern of the revertants if completely different from that in the wild type strain. A discussion of possible mechanisms of general control of amino acid biosynthesis in H. henricii is presented.