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Related Experiment Videos

The development of cell junction during nephrogenesis

M Minuth, A Schiller, R Taugner

    Anatomy and Embryology
    |January 1, 1981
    PubMed
    Summary

    Rabbit nephron development involves the formation of tight junctions, crucial for establishing cellular barriers. These intercellular junctions differentiate from focal connections to continuous seals, aligning with lumen formation and glomerular filtration onset.

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    Area of Science:

    • Nephrology
    • Developmental Biology
    • Cell Biology

    Background:

    • Intercellular junctions, particularly tight junctions (zonulae occludentes), play a critical role in epithelial barrier function.
    • Understanding their differentiation during early nephrogenesis is essential for comprehending kidney development and potential pathologies.

    Purpose of the Study:

    • To investigate the differentiation and distribution of intercellular junctions during early rabbit nephron development.
    • To characterize the ultrastructural changes in tight junctions throughout nephrogenesis.

    Main Methods:

    • Freeze-fracture electron microscopy was employed to visualize the ultrastructure of intercellular junctions.
    • The study focused on metanephrogenic cells, renal vesicles, S-shaped bodies, and glomerular epithelia in rabbit kidneys.

    Main Results:

    • Sporadic focal tight junctions connect metanephrogenic cells, evolving into broad zonulae occludentes lining the lumen of renal vesicles and S-shaped bodies.
    • Early tight junctions exhibit beaded rows of particles, transitioning to continuous junctions with varying strand numbers across nephron segments.
    • Visceral glomerular cells show a reduction of occluding zonules to maculae occludentes, eventually disappearing, unlike parietal glomerular epithelium.

    Conclusions:

    • Zonulae occludentes formation is synchronous with lumen establishment in the developing nephron.
    • Tight junction ultrastructural differentiation appears complete by the onset of glomerular filtration.

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