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Cell and virus propagation on cylindrical cellulose based microcarriers
Developments in Biological Standardization
|January 1, 1981
Summary
Microgranular DEAE-cellulose microcarriers support anchorage-dependent cell culture, including established and primary cells, for similar yields to commercial systems. Virus propagation on these cells also matched conventional monolayer cultures.
Area of Science:
- Biotechnology
- Cell Biology
- Biomaterials
Background:
- Anchorage-dependent cells require solid surfaces for growth.
- Microcarrier technology offers a scalable solution for cell culture.
- DEAE-cellulose is an anion exchanger with potential for cell adhesion.
Purpose of the Study:
- To evaluate microgranular DEAE-cellulose as a microcarrier for anchorage-dependent cell culture.
- To determine optimal exchange capacities for different cell types.
- To assess cell yields and virus propagation efficiency.
Main Methods:
- Testing microgranular DEAE-cellulose with varying exchange capacities (0-2.7 meq/gr).
- Culturing established cell lines (BHK, MDCK), primary cells (chick embryo fibroblast), and human diploid cell strains.
- Comparing cell yields and virus propagation to commercial microcarriers and conventional monolayers.
Main Results:
- Established cell lines formed confluent monolayers on DEAE-cellulose with 1.00-2.0 meq/gr capacity.
- Primary cells formed cell-microcarrier aggregates on DEAE-cellulose with 1.59-2.0 meq/gr capacity.
- Cell yields and virus propagation were comparable to DEAE-dextran microcarriers and conventional cultures.
Conclusions:
- Microgranular DEAE-cellulose is a suitable microcarrier for culturing specific anchorage-dependent cells.
- Optimized exchange capacities facilitate different growth patterns (monolayer vs. aggregate).
- DEAE-cellulose microcarriers offer a viable alternative for cell culture and virus propagation.