Glyceraldehyde-3-phosphate dehydrogenase from human muscle: primary structure

The complete sequence of 334 amino acids of glyceraldehyde-3-phosphate dehydrogenase (GPDH) from human muscle was established through the analysis of tryptic digests of the enzyme treated alternatively with bromocyanate, o-iodosobenzoic acid and maleic acid. Comparison of the primary structure of the enzyme from human muscle with that of the other known GPDH showed high homology; the closest similarity of the amino acid sequence was found between the enzymes from human and pig muscle.