Related Experiment Videos
Circulating antigens in ovine haemonchosis
Annales De Recherches Veterinaires. Annals of Veterinary Research
|January 1, 1981
Summary
A new sandwich ELISA test can detect Haemonchus contortus antigens. However, circulating antigens were not generally found in serum, urine, or milk of infected ewes, suggesting it may not be a reliable diagnostic marker for ovine haemonchosis.
Area of Science:
- Veterinary Parasitology
- Immunodiagnostics
- Sheep Health
Background:
- Haemonchus contortus is a significant gastrointestinal nematode parasite affecting sheep globally.
- Accurate diagnostics are crucial for managing ovine haemonchosis.
- Detection of parasite antigens in host fluids could offer a non-invasive diagnostic approach.
Purpose of the Study:
- To develop and validate a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for Haemonchus contortus antigens.
- To investigate the presence of H. contortus circulating antigens in serum, urine, and milk of experimentally infected ewes.
Main Methods:
- Development of a sandwich ELISA specific for H. contortus antigens.
- Experimental infection of ewes with H. contortus.
- Assay of serum, urine, and milk samples from infected ewes using the developed sandwich ELISA.
Main Results:
- The sandwich ELISA demonstrated sensitive detection of H. contortus antigens.
- Circulating antigens were not consistently detected in the serum, urine, or milk of experimentally infected ewes.
- The absence of general antigenemia/antigenuria/antigenilactia suggests limitations for this diagnostic approach.
Conclusions:
- While a sensitive sandwich ELISA was developed, circulating H. contortus antigens are not a reliable indicator in all tested body fluids of infected ewes.
- Ovine haemonchosis diagnosis may require alternative or complementary methods.
- Further research is needed to explore other potential biomarkers or refine antigen detection strategies.