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Related Experiment Videos

Experimental data in keratophakia

F Hoffmann

    Developments in Ophthalmology
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    This study measured corneal stroma temperature during keratophakia using a thermistor. Cryoprotective agents reduced radioactivity by 30-50% compared to non-frozen controls, indicating potential tissue viability.

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    Area of Science:

    • Ophthalmology
    • Cryobiology
    • Biomedical Engineering

    Background:

    • Keratophakia involves freezing corneal tissue.
    • Accurate temperature monitoring during freezing is crucial for tissue preservation.
    • Cryoprotective agents are used to minimize cellular damage during cryopreservation.

    Purpose of the Study:

    • To measure the temperature decrease in corneal stroma during keratophakia.
    • To program a constant rate freezer for reproducible freezing curves.
    • To assess the impact of cryoprotective agents on tissue viability post-freezing.

    Main Methods:

    • Utilized a thermistor to monitor temperature changes in the corneal stroma.
    • Employed a constant rate freezer programmed with a specific temperature profile.

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  • Measured radioactivity after 35S incorporation to assess cell viability in frozen and non-frozen corneas.

    • Main Results:

    • Corneal stroma temperature reached -15°C at 1 minute, -40°C at 2 minutes, and -50°C at 4 minutes after initiating freezing.
    • The constant rate freezer allowed for simultaneous freezing of multiple tissue samples with reproducible freezing curves.
    • Corneas treated with cryoprotective agents showed 50-70% of the radioactivity of non-frozen control corneas, suggesting partial preservation of cellular function.

    Conclusions:

    • The study successfully monitored and programmed corneal stroma freezing temperatures for keratophakia.
    • Cryoprotective agents appear to offer partial protection to corneal tissue during the freezing process.
    • Further research is warranted to optimize cryopreservation protocols for keratophakia to improve tissue viability.