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The relationship between the minimal and the 50% haemolytic dose in complement titrations
The Onderstepoort Journal of Veterinary Research
|December 1, 1981
Summary
The 50% spectrophotometric complement titration end-point (C'H50) offers greater reproducibility than the 100% end-point (MHD). Thrombin tubes accelerate complement preparation, completing it within an hour for improved laboratory efficiency.
Area of Science:
- Immunology
- Biochemistry
Background:
- Complement titration is crucial for assessing immune function.
- Standardized methods are needed for reproducible complement assays.
Purpose of the Study:
- To compare the reproducibility of 50% and 100% spectrophotometric complement titration end-points.
- To establish the relationship between the 100% end-point (MHD) and the 50% end-point (C'H50).
- To evaluate the efficiency of complement preparation using thrombin tubes.
Main Methods:
- Spectrophotometric complement titration was performed using both 50% (C'H50) and 100% (MHD) end-points.
- The relationship between MHD and C'H50 was determined.
- Complement preparation was assessed using thrombin tubes for rapid clotting.
Main Results:
- The 50% end-point (C'H50) demonstrated higher reproducibility compared to the 100% end-point (MHD).
- A consistent ratio of 1:2.5 was observed between the MHD and C'H50.
- Thrombin tubes facilitated clotting within 2 minutes, enabling complement preparation in under 1 hour.
Conclusions:
- The C'H50 assay provides a more reproducible measure of complement activity.
- The established MHD to C'H50 ratio aids in standardizing results.
- Thrombin tubes significantly expedite the complement preparation process, enhancing laboratory workflow.