[Puromycin action on intestinal lipid absorption in male and female rats]

The effect of puromycin on absorption balance and mucosal lipid distribution was studied during intestinal absorption of 14C oleic acid and 3H palmitic acid, using the isolated intestinal loop of male and female rats in situ. In puromycin-treated animals, mucosal lipid uptake was reduced and mucosal lipid content increased, particularly with oleic acid and in male rats. The phospholipids were significantly reduced. As revealed by mucosal accumulation of free fatty acids, esterification was particularly inhibited with oleic acid and in female rats. These results might explain why portal blood radioactivity was higher in treated rats than in the controls. Puromycin effect occurred at different stages of lipid absorption and varied with sex.