[Role of leukocytes in the healing process in surgical wounds]

In the last period of time there has been stated that in human lysosomes of neutrophilic leukocytes an in ones of some experimental animals occur, which stimulate the growth of lymphocytes and fibroblasts in the study in vitro. With regard to this it was assumed that similar stimulating processes may take place in the surgical wounds. The experiments were carried out on guinea pigs which were wounded and afterwards the wounds were sutured and the allogenic proteins preparations were introduced into its in dose of 100 microgram as follows: lysosomal proteins of granulocytes, mixed and separated on the the DEAE-cellulose columns; lysosomal proteins of macrophages, the proteins isolated from plasma and nuclei both the granulocytes and macrophages. The evaluation of healing was based on the measurement of mechanical resistance of wounds adhesions and was completed with the pathomorphological examinations. It has been shown that the mixture of lysosomal proteins of granulocytes, intruduced into the wounds, caused the acceleration of the healing processes. After giving into the wounds some fractions of proteins which were separated on the colums with DEAE-cellulose, the stimulating processes of healing were seen, while after adding the other ones-the inhibiting processes. The protein with similar evidences were not show inside other structures studied of granulocytes and macrophages. The results obtained seem to support the supposition that in lysosomes of neutrophilic granulocytes there are the factors which regulate the repair processes in wounds.