Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Calcification by proteolipid from atherosclerotic aorta

J Ennever, J J Vogel, L J Riggan

Atherosclerosis

|February 1, 1980

Summary

Researchers identified a proteolipid in calcified atherosclerotic aorta that can initiate apatite crystal formation, the primary component of aortic calcification. This finding suggests a potential mechanism for vascular calcification similar to bone development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

'A time bomb ticking in my head': drawings of inner ears by patients with vestibular schwannoma.

Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery·2011

Same author

Pathogenicity of the pulpless tooth.

Washington University dental journal·2010

Same author

Redesigning nucleic acids.

Pure and applied chemistry. Chimie pure et appliquee·2001

Same author

1,25 dihydroxyvitamin D(3) activates sphingomyelin turnover in ROS17/2.8 osteosarcoma cells without sphingolipid-induced changes in cytosolic Ca(2+).

Biochemical and biophysical research communications·2000

Same author

Fingertip necrosis during chemotherapy with bleomycin, vincristine and methotrexate for HIV-related Kaposi's sarcoma.

The British journal of dermatology·1996

Same author

Mineralization of bone-like extracellular matrix in the absence of functional osteoblasts.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·1995

Area of Science:

Biochemistry
Cardiovascular Research
Mineralization

Background:

Aortic calcification is a hallmark of atherosclerosis, contributing to cardiovascular disease.
The specific mechanisms driving apatite crystal formation in the aorta remain incompletely understood.

Purpose of the Study:

To investigate the presence and role of a proteolipid in initiating apatite nucleation within calcified atherosclerotic aorta.
To characterize the identified proteolipid and compare its function to known nucleators in biomineralization.

Main Methods:

Decalcification of aortic tissue using dilute formic acid.
Extraction and lipid fractionation to isolate proteolipid.
Incubation of the isolated proteolipid in a metastable calcium phosphate solution to observe apatite induction.

Main Results:

A proteolipid capable of nucleating apatite was successfully isolated from calcified atherosclerotic aorta.
The isolated proteolipid was characterized as a complex of hydrophobic protein and acidic phospholipid.
This proteolipid demonstrated the ability to induce apatite crystallization in vitro.

Conclusions:

A specific proteolipid acts as a nucleator for apatite formation in the aorta, mirroring processes in bone calcification.
Understanding this mechanism could offer new insights into the pathogenesis of vascular calcification.

Related Experiment Videos