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Related Concept Videos

Disassembly of Intermediate Filaments01:35

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Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
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In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
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Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
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Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
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The nuclear envelope lamina is reversibly depolymerized during mitosis

L Gerace, G Blobel

    Cell
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    The nuclear lamina reversibly disassembles during cell division. Mitotic lamins A, B, and C depolymerize, becoming monomeric and more phosphorylated, suggesting phosphorylation controls lamina disassembly.

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    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • The nuclear lamina, a protein structure on the inner nuclear membrane, comprises lamins A, B, and C.
    • Previous work showed lamina disassembly during cell division coincides with nuclear envelope reconstruction.

    Purpose of the Study:

    • To investigate the molecular mechanisms of nuclear lamina disassembly during cell division.
    • To determine the state and properties of lamins during mitosis.

    Main Methods:

    • Immunofluorescence microscopy
    • Cell fractionation
    • Immunoprecipitation studies
    • Sucrose gradient sedimentation analysis
    • Quantitative immunoprecipitation

    Main Results:

    • Lamins A and C are soluble and non-membrane-associated during mitosis.
    • Mitotic lamin B may associate with membrane fragments.
    • All three lamins exist as monomers during mitotic disassembly.
    • Mitotic lamins exhibit increased phosphorylation and a more acidic isoelectric point.

    Conclusions:

    • The nuclear lamina undergoes reversible depolymerization during cell division.
    • Enzymatic phosphorylation of lamins likely regulates their polymerization state and mitotic disassembly.