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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

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Ionised calcium in pathological human bile

D J Sutor, L I Wilkie, M J Jackson

Journal of Clinical Pathology

|January 1, 1980

Summary

This summary is machine-generated.

This study measured ionized calcium in bile using an automated system. Results show a strong correlation between ionized and total calcium levels in patients with gallstones.

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Area of Science:

Biochemistry
Medical Diagnostics

Background:

Gallstones are a common condition.
Calcium plays a role in bile composition and gallstone formation.

Purpose of the Study:

To measure ionized calcium concentrations in human bile.
To investigate the relationship between ionized calcium and total calcium in bile.

Main Methods:

An automated ion-selective electrode system was employed.
Bile samples were collected from patients undergoing cholecystectomy for gallstones.
Ionized calcium and total calcium levels were quantified.

Main Results:

Ionized calcium in bile ranged from 0.63 to 1.73 mmol/l.
Total calcium concentrations varied from 1.35 to 10.93 mmol/l.
A highly significant positive correlation (r = 0.97) was observed between ionized and total calcium.

Conclusions:

Automated measurement of ionized calcium in bile is feasible.
Ionized calcium levels in bile are strongly correlated with total calcium.
These findings may contribute to understanding calcium's role in gallstone disease.