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Related Experiment Videos

Pre-precipitated and solid-phase second antibody compared in radioimmunoassay

T R Brown, N Bagchi, T T Ho

    Clinical Chemistry
    |March 1, 1980
    PubMed
    Summary

    Pre-precipitated second antibodies offer a cost-effective and efficient alternative for radioimmunoassays. They improve assay precision and reduce nonspecific binding compared to commercial solid-phase antibodies.

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    Area of Science:

    • Immunochemistry
    • Biochemical Assays

    Background:

    • Second antibodies are crucial for separating antibody-bound antigens in immunoassays.
    • Commercial solid-phase second antibodies (DASP) are widely used but can be expensive.

    Purpose of the Study:

    • To present simple methods for preparing and titering pre-precipitated second antibodies.
    • To compare the performance of pre-precipitated second antibodies with commercial DASP.

    Main Methods:

    • Preparation and titration of insolubilized second antibodies.
    • Comparative analysis of pre-precipitated and DASP reagents in reverse triiodothyronine (rT3) and thyrotropin radioimmunoassays.

    Main Results:

    • Equilibrium time was reduced to approximately 30 minutes for both reagent types.

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  • Pre-precipitated second antibodies demonstrated improved within-assay precision and lower nonspecific binding.
  • Cost analysis revealed pre-precipitated antibodies to be 3-10 times less expensive than DASP.

    • Conclusions:

    • Pre-precipitated second antibodies provide a simpler, more precise, and cost-effective alternative for radioimmunoassays.
    • These findings support the adoption of pre-precipitated second antibodies in routine laboratory practices.