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Immunoreactive calcitonin in human cerebrospinal fluid
The Journal of Clinical Endocrinology and Metabolism
|April 1, 1980
Summary
Immunoreactive calcitonin (CT) was detected in 75% of male subjects' cerebrospinal fluid (CSF) using radioimmunoassay (RIA). CSF CT levels did not correlate with plasma CT, and its source remains undetermined.
Area of Science:
- Neuroendocrinology
- Biochemistry
- Clinical Chemistry
Background:
- Calcitonin (CT) is a hormone primarily known for its role in calcium regulation.
- The presence and source of calcitonin within the central nervous system, specifically cerebrospinal fluid (CSF), are not well-established.
Purpose of the Study:
- To investigate the presence and characteristics of immunoreactive calcitonin in human cerebrospinal fluid (CSF).
- To explore potential correlations between CSF calcitonin and plasma calcitonin levels.
Main Methods:
- Radioimmunoassay (RIA) was employed to quantify immunoreactive calcitonin (CT) in the cerebrospinal fluid (CSF) of 123 male subjects.
- Simultaneous measurements of CSF and plasma CT were performed in a subset of subjects.
- Column chromatography was utilized to characterize the immunoreactivity in pooled CSF samples.
Main Results:
- Immunoreactive CT was detected in 75% of the studied CSF samples, with a mean concentration of 11.1 pg/ml.
- No significant correlation was found between cerebrospinal fluid (CSF) calcitonin and plasma calcitonin levels.
- Chromatographic analysis indicated that the majority of CSF CT immunoreactivity behaved similarly to authentic human CT.
Conclusions:
- This study confirms the presence of immunoreactive calcitonin in human cerebrospinal fluid.
- The findings suggest that CSF calcitonin may not be directly derived from plasma CT.
- The precise origin of calcitonin within the CSF requires further investigation.