[Chromatographic analysis of the hemin isolated from the erythocytes of hematological patients]

Fractional composition of hemin, isolated from blood of patients with hematological impairments, differed from the composition characteristic for donors as shown by paper chromatography. Three fractions of hemin, which corresponded to various steps of polymerization of the substance, were found on chromatograms of donor hemin. Hemin from the patients were characterized by alteration both in amount and in the ratio of the fractions. Estimation of the hemin composition using paper chromatography might serve as an additional diagnostic test.