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Related Experiment Videos

Basis of phencyclidine's ability to decrease the synaptosomal accumulation of 3H-catecholamines

T E Ary, H L Komiskey

    European Journal of Pharmacology
    |February 1, 1980
    PubMed
    Summary

    Phencyclidine (PCP) likely hinders norepinephrine uptake in brain synaptosomes. However, PCP may also trigger dopamine release from these same brain cells, impacting dopamine accumulation.

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    Area of Science:

    • Neuroscience
    • Pharmacology
    • Biochemistry

    Background:

    • Synaptosomes are crucial for neurotransmitter uptake and release.
    • Phencyclidine (PCP) is a known psychoactive substance with complex neurochemical effects.
    • Understanding PCP's interaction with monoamine transporters is vital for neuroscience research.

    Purpose of the Study:

    • To investigate the effects of phencyclidine (PCP) on the accumulation of norepinephrine and dopamine in specific brain regions.
    • To differentiate between PCP's inhibition of neurotransmitter uptake and its potential to induce neurotransmitter release.

    Main Methods:

    • Incubation of mixed cortical and hypothalamic synaptosomes with 3H-norepinephrine.
    • Incubation of striatal synaptosomes with 3H-dopamine.

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  • Assessment of monoamine accumulation in the presence and absence of phencyclidine (PCP).

    • Main Results:

    • Phencyclidine (PCP) significantly decreased the accumulation of 3H-norepinephrine in cortical and hypothalamic synaptosomes, suggesting blockade of amine uptake.
    • A 10 muM concentration of PCP partially reduced 3H-dopamine accumulation in striatal synaptosomes, indicating both uptake inhibition and release of previously stored dopamine.

    Conclusions:

    • Phencyclidine (PCP) exhibits differential effects on norepinephrine and dopamine transport systems.
    • PCP's mechanism involves blocking norepinephrine uptake while also promoting dopamine release from synaptosomes.