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ATP dependence of H+ secretion
The Journal of Cell Biology
|May 1, 1980
Summary
Adenosine triphosphate (ATP) can fuel acid secretion in rabbit gastric parietal cells. Electrical shock impairs acid production, but ATP can restore this function, suggesting it acts as a direct energy substrate.
Area of Science:
- Physiology
- Cell Biology
- Biochemistry
Background:
- Parietal cells are responsible for gastric acid secretion.
- The energy requirements for acid secretion are not fully understood.
- Adenosine triphosphate (ATP) is a key cellular energy currency.
Purpose of the Study:
- To investigate the role of ATP in gastric acid secretion.
- To determine if ATP can directly provide energy for proton (H+) secretion in parietal cells.
Main Methods:
- Rabbit gastric glands were made permeable to ATP using high voltage discharge.
- Acid production was assessed by acridine orange accumulation and [14C]aminopyrine accumulation.
- Mitochondrial respiration was inhibited using cyanide, azide, or Amytal.
Main Results:
- Electrical shock reduced acid production in parietal cells.
- ATP restored acid secretion in shocked glands, particularly in high potassium conditions.
- ATP addition to shocked glands with inhibited mitochondrial respiration rescued acid secretion.
Conclusions:
- ATP can serve as a direct substrate for proton (H+) secretion in gastric parietal cells.
- This suggests that ATP may be sufficient as an energy source for acid secretion, potentially independent of mitochondrial respiration.