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Human peripheral Nullymphocytes: ultrastructural aspects of the "K" cell effect against a melanoma target cell
A null cell fraction was isolated from human peripheral blood by Ficoll density gradient centrifugation followed by removal of mononuclear phagocytes, passage through Ig-antiIg columns and sedimentation of E-rosette forming T cells. In contrast to the T cell fraction in the Null cell population exhibited cytotoxic activity against antibody-sensitized human melanoma target cells (ADCC reaction). This activity could be attributed to a low affinity Fc-receptor bearing cells present in the Null cell preparation. At the ultrastructural level in 2 h ADCC reaction most tumor cells were surrounded by up to 10 lymphoid cells which showed narrow junctions with parallel plasma membranes and local evaginations of lymphoid cells into recessus of the target melanoma cells. The mononuclear cell type found in close contact with the antibody-sensitized target cells had the following morphological criteria: 8 to 10 mu diameter, irregular nucleus, discrete rough endoplasmic reticulum, isolated ribosomes, cytoplasmic organelles concentrated under a ruffling membrane in the contact area with the tumor cell.
A null cell fraction was isolated from human peripheral blood by Ficoll density gradient centrifugation followed by removal of mononuclear phagocytes, passage through Ig-antiIg columns and sedimentation of E-rosette forming T cells. In contrast to the T cell fraction in the Null cell population exhibited cytotoxic activity against antibody-sensitized human melanoma target cells (ADCC reaction). This activity could be attributed to a low affinity Fc-receptor bearing cells present in the Null cell preparation. At the ultrastructural level in 2 h ADCC reaction most tumor cells were surrounded by up to 10 lymphoid cells which showed narrow junctions with parallel plasma membranes and local evaginations of lymphoid cells into recessus of the target melanoma cells. The mononuclear cell type found in close contact with the antibody-sensitized target cells had the following morphological criteria: 8 to 10 mu diameter, irregular nucleus, discrete rough endoplasmic reticulum, isolated ribosomes, cytoplasmic organelles concentrated under a ruffling membrane in the contact area with the tumor cell.