Comparison of the carcinoembryonic antigen levels by dialysis and sephadex column methods

Duplicate plasma samples from 64 human donors were tested and compared for the levels of carcinoembryonic antigen (CEA) by the conventional dialysis and sephadex column methods. Results from the column method were similar to those from the dialysis method, with a correlation coefficient of 0.9959. In addition, the assay time by the sephadex column was only four to five hours, as compared with 18 to 24 hours for the dialysis procedure. The potential usefulness in substituting the sephadex column for the dialysis method in CEA determinations is discussed.