Electrophoretic properties and specificity of human gastric lipase

Electrophoretic separation of gastric juice and the homogenate of stomach body mucosa on polyacrylamide gel followed by staining for esterolytic activity resulted in a characteristic zymogram composed of five activity bands located in the cathodal part of the electropherogram. This pattern was lacking in zymograms obtained for the homogenates of antral and duodenal mucosa. Lipolytic activity was confined to the same area of the gel where the five band pattern of esterolytic activity was localized. It was concluded that gastric juice and homogenate of stomach body mucosa contain one esterolytic enzyme of wide specificity, known as gastric lipase.