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Related Experiment Videos

Smear platelet counts

A Bell, C L Neely

    Southern Medical Journal
    |July 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Estimating platelet counts using a multiplication factor of 20,000 is reliable for normal and low platelet levels. This method becomes less accurate when platelet counts are very high (thrombocytosis).

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    Area of Science:

    • Hematology
    • Clinical Pathology

    Background:

    • Accurate platelet quantification is crucial for diagnosing and managing bleeding disorders.
    • Manual blood smear examination remains a common method for platelet counting.

    Purpose of the Study:

    • To evaluate the reliability of a specific manual platelet counting method.
    • To establish a practical estimation technique for platelet counts.

    Main Methods:

    • Counting the average number of platelets across ten oil immersion fields.
    • Multiplying the average count by a factor of 20,000.
    • Statistical analysis of 288 unselected patients to determine the multiplication factor.

    Main Results:

    • The multiplication factor of 20,000 provides a reliable platelet count estimation in normal and thrombocytopenic states.

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  • The method's reliability decreases with thrombocytosis (≥25 platelets per oil immersion field).

    • Conclusions:

    • A simple multiplication factor of 20,000 offers a dependable estimation for platelet counts in most clinical scenarios.
    • Careful attention to sample quality, smear preparation, and staining is essential for accurate results.