[Effect of bicarbonate and certain anions on NADP redox]

The paper deals with the intensity of NADPH formation in the isocytrate dehydrogenase reaction and NADPH oxidation in the lactate and malate dehydrogenase reactions. It is shown that bicarbonate, phosphate, acetate, chloride and triethanolamine buffer in concentrations of 1 . 10(-1) = 4 . 10(-1) M can inhibit these processes intensity. The inhibitory effect of the mentioned anions on the NADH-isocytrate dehydrogenase activity is considerably decreased when adding MnCl2 or MgCl2 to the incubation medium. Sucrose is established to inhibit the formation of NADPH in the isocitrate dehydrogenase reaction at 2 . 10(-1) M and higher.