Cryopreservation of cells for immunological typing of non-Hodgkin's lymphomas

The influence of cryopreservation on markers and functions of cells from lymph node, spleen, and peripheral blood of patients with non-Hodgkin's lymphoma was investigated. For the markers and function tested, there were good correlations between the values obtained with fresh cells and those obtained with cryopreserved cells, proveded that cryopreserved cells were incubated for at least 3 hr at 37 degrees prior to testing.