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[Autoradiographic detection of tritiated choline chloride in brain tissue. A method of microscopic detection in
Journal Fur Hirnforschung
|January 1, 1978
Abstract:
It is presented a autoradiographic method for microscopical detection of 3H-choline chloride activity after intraventricular application in freeze-dried and paraffin-embedded brain sections. The 10 micrometer thick sections are treated with diethyl ether and than exposed during 90 days above calcium chloride at 4 degree C using the sandwich technique (with stripping film). After separate film explanation and section staining the film and the brain sections are mounted together.