Related Experiment Video
Updated: Jul 18, 2026
09:46
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
[Autoselection of Pichia pinus yeasts by 3-stage multiflow cultivation]
Mikrobiologiia
|March 1, 1980
Abstract:
Three-stage multiflow cultivation of the yeast Pichia pinus, with the flow rates of 0.27, 0.6 and 1.2 hr-1, respectively, in the first, second and third fermenters, resulted in autoselection and the parent strain was substituted by a selective one in the third fermenter. The selective strain differed from the parent one by the size of cells, had a higher specific growth rate, and contained more protein. These properties of the selective strain were preserved in the course of one-stage cultivation.
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