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Practical anaerobic broth-disk elution susceptibility test

J H Jorgensen, G A Alexander, J E Johnson

Antimicrobial Agents and Chemotherapy

|April 1, 1980

Summary

A modified broth-disk elution method offers a practical approach for routine anaerobic bacteria susceptibility testing in clinical labs. This method shows favorable comparisons with established techniques for most antibiotics tested.

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Area of Science:

Clinical Microbiology
Antimicrobial Susceptibility Testing
Anaerobic Bacteriology

Background:

Accurate antimicrobial susceptibility testing (AST) is crucial for guiding treatment of anaerobic bacterial infections.
Standardized methods for anaerobic AST are essential for reliable clinical laboratory results.
The National Committee on Clinical Laboratory Standards (NCCLS) proposed a reference agar dilution method for anaerobic bacteria AST.

Purpose of the Study:

To evaluate a modified broth-disk elution method for routine susceptibility testing of anaerobic bacteria.
To compare the performance of the modified broth-disk elution method against the proposed NCCLS reference agar dilution method.
To assess the practicality and reliability of the broth-disk elution method for clinical microbiology laboratories.

Main Methods:

A modified broth-disk elution method was developed using Schaedler broth medium with menadione and GasPak incubation.
A 24-hour incubation period was employed for the broth-disk elution method.
Results were compared with the proposed NCCLS reference agar dilution method for several antibiotics.

Main Results:

The modified broth-disk elution method demonstrated favorable agreement with the reference method for carbenicillin, chloramphenicol, clindamycin, and penicillin.
A 24-hour incubation period resulted in significant discrepancies specifically for tetracycline disk elution tests.
The method proved practical for routine testing of anaerobic bacterial isolates.

Conclusions:

The modified broth-disk elution method is a viable and practical option for routine anaerobic bacteria susceptibility testing in clinical settings.
While generally reliable, the 24-hour incubation period requires careful consideration for certain antibiotics like tetracycline.
This method can aid hospital clinical microbiology laboratories in efficiently identifying effective antibiotic treatments for anaerobic infections.

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