Related Experiment Videos
Phagocytic activity of human polymorphonuclear leukocytes at different temperatures
Abstract:
Phagocytic activity from human PMN obtained from defibrinated peripheral blood of young adults of both sexes was studied. Defibrinated blood was distributed in volumes of 0.6 ml over 12 glass slip covers, and incubated at 37 degrees C in humid chamber for 30 minutes, in order to permit the PMN adherence to glass. Then the PMN were rinsed with culture medium, covered with 0.6 ml of 1 x 10(6) latex particles (0.81 microns, Bacto-latex Difco) suspension in autologous serum by milliliter, and incubated by triplicate per 30 minutes at 35 degrees C, 37 degrees C and 39 degrees C. Then slip covers were rinsed with culture medium and stained with Wright stain. Phagocytic index was calculated as the average of phagocytized latex particles per 300 PMN. The results obtained were: at 35 degrees C, 17.26 +/- 6.1; at 37 degrees C, 19.80 +/- 6.26; at 39 degrees C, 21.94 +/- 6.94; and at 41 degrees C, 25.58 +/- 6.69. The correlation coefficient between this temperature increase and the phagocytic indexes was 0.41. This allow to conclude that a direct linear increment exists between the PMN phagocytic indexes and the temperature increase.
Insights
Increasing temperature enhances the phagocytic activity of human polymorphonuclear leukocytes (PMN). Higher temperatures lead to a greater number of latex particles being engulfed by PMN, indicating improved immune cell function.
Area of Science:
- Immunology
- Cellular Biology
Background:
- Polymorphonuclear leukocytes (PMN) are crucial immune cells involved in phagocytosis.
- Understanding factors influencing PMN phagocytic activity is vital for immune response studies.
Purpose of the Study:
- To investigate the effect of temperature on the phagocytic activity of human PMN.
- To quantify the relationship between temperature and PMN phagocytic index.
Main Methods:
- Human PMN were isolated from defibrinated blood.
- PMN were incubated with latex particles at various temperatures (35°C, 37°C, 39°C, 41°C).
- Phagocytic index was determined by counting engulfed particles per 300 PMN.
Main Results:
- Phagocytic activity increased with temperature.
- At 35°C, the index was 17.26; at 37°C, 19.80; at 39°C, 21.94; and at 41°C, 25.58.
- A direct linear correlation (r=0.41) was observed between temperature and phagocytic index.
Conclusions:
- Elevated temperatures significantly enhance PMN phagocytic activity.
- Temperature is a key factor influencing the efficiency of phagocytosis by human PMN.