Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in bilirubins in human prenatal development

S G Blumenthal, T Stucker, R D Rasmussen

    The Biochemical Journal
    |March 15, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Therapeutic targeting of constitutive PARP activation compromises stem cell phenotype and survival of glioblastoma-initiating cells.

    Cell death and differentiation·2013
    Same author

    Characteristics of nucleic acids.

    Current protocols in nucleic acid chemistry·2008
    Same author

    Palladium-mediated C5 substitution of pyrimidine nucleosides.

    Current protocols in nucleic acid chemistry·2008
    Same author

    Unnatural nucleosides with unusual base pairing properties.

    Current protocols in nucleic acid chemistry·2008
    Same author

    Validation of large-scale, monochromatic UV disinfection systems for drinking water using dyed microspheres.

    Water research·2007
    Same author

    Importance of ets1 proto-oncogene for breast cancer progression.

    Zentralblatt fur Gynakologie·2004

    This study developed a method to quantify fetal bilirubin levels in bile and meconium. Bilirubin composition in fetal bile changes significantly during gestation, with unconjugated bilirubin-IX alpha becoming dominant near term.

    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Analytical Chemistry

    Background:

    • Bile pigments, primarily bilirubin, play crucial roles in fetal development and are indicators of physiological processes.
    • Understanding the ontogeny of bilirubin metabolism in fetuses is essential for diagnosing and managing neonatal conditions.

    Purpose of the Study:

    • To develop and validate a densitometric method for quantifying azodipyrroles derived from bile pigments.
    • To investigate the changes in bilirubin composition in fetal bile and meconium during gestation (14-36 weeks).

    Main Methods:

    • A novel densitometric method was established using diazotized ethyl anthranilate for azodipyrrole quantification.
    • Bile and meconium samples from human fetuses across a gestational range were analyzed.

    Related Experiment Videos

    Main Results:

    • Bile pigments were undetectable before 14 weeks of gestation.
    • Bilirubin-IX beta was the primary pigment from 14-20 weeks, with early traces of unconjugated bilirubin-IX alpha appearing at 16-17 weeks.
    • Significant shifts occurred around 20 weeks, with the appearance of bilirubin-IX alpha monoconjugates and glucuronides, becoming predominant by term.

    Conclusions:

    • The developed densitometric method accurately quantifies fetal bile pigments.
    • Fetal bile bilirubin composition undergoes dynamic changes throughout gestation, reflecting developmental maturation of metabolic pathways.
    • The transition from bilirubin-IX beta to conjugated bilirubin-IX alpha derivatives highlights key developmental milestones in bilirubin metabolism.