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Related Experiment Videos

Nucleolar changes in senescing WI-38 cells

P M Bemiller, L H Lee

    Mechanisms of Ageing and Development
    |December 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    As WI-38 fibroblast cells age in vitro, their nucleoli significantly enlarge and increase in dry mass. This study documents these key cellular changes during senescence.

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    Area of Science:

    • Cell Biology
    • Aging Research
    • Biophysics

    Background:

    • Cellular senescence is a complex process associated with aging.
    • Nucleoli are dynamic nuclear structures involved in ribosome biogenesis and cellular stress responses.
    • Understanding nucleolar changes during senescence is crucial for aging research.

    Purpose of the Study:

    • To investigate alterations in nucleolar size, dry mass, and morphology during in vitro aging of WI-38 cells.
    • To quantify the changes in nucleolar characteristics as cells progress through senescence.
    • To establish correlations between nucleolar dry mass and area.

    Main Methods:

    • Utilized WI-38 fibroblast cell cultures.
    • Employed in vitro aging models to induce senescence.

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  • Applied interferometric methods for precise nucleolar dry mass determination.
  • Analyzed nucleolar area and morphology changes.

    • Main Results:

    • Observed a significant increase in the proportion of cells with a single, enlarged nucleolus (from 17% to 93%).
    • Documented a substantial rise in mean nucleolar dry mass (583%) and mean nucleolar area (236%) by the final doubling.
    • Established a strong positive correlation (r = 0.92) between nucleolar dry mass and nucleolar area.

    Conclusions:

    • In vitro senescence of WI-38 cells is characterized by significant nucleolar hypertrophy and increased dry mass.
    • Nucleolar size and mass are reliable indicators of cellular aging in this model.
    • The strong correlation suggests a coupled regulation of nucleolar mass and volume during senescence.