[Progression of new transplantable hepatomas in C3HA strain mice. I. 61EP hepatomas]

Three new morphologically different strains of stabilized transplantable hepatomas in C3HA mice were obtained using continuous transplantation of nitrosodiethylamine induced primary liver tumour by the method of parallel sublines. Morphology and development of biological properties of substrains of hepatomas are described. All differences between substrains arose gradually throughout a number of generations, with malignization of hepatomas occurring. If the development of hepatomas was accompanied with differentiation, as was the case in some substrains in our experiments, the rate of a successful transplantation diminished, and it was impossible to obtain stabilized strains of transplantable hepatomas.