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Separation of alphavirus nucleocapsids from envelope fragments
Zeitschrift Fur Naturforschung. Section C, Biosciences
|January 1, 1980
Summary
Formaldehyde treatment stabilizes fragile alphavirus nucleocapsids and envelope fragments. This method enables detailed biochemical, electron microscopic, and serological studies of alphavirus structure and function.
Area of Science:
- Virology
- Structural Biology
- Biochemistry
Background:
- Alphavirus nucleocapsids are fragile and prone to disruption during isolation.
- Previous methods hindered morphological studies due to particle instability.
Purpose of the Study:
- To describe methods for preparing alphavirus nucleocapsids and envelope fragments.
- To overcome challenges in isolating intact viral particles for investigation.
Main Methods:
- Preparation of alphavirus nucleocapsids and envelope fragments.
- Treatment with formaldehyde to stabilize viral particles.
Main Results:
- Formaldehyde treatment successfully prevented disruption of fragile alphavirus particles.
- Stable nucleocapsids and envelope fragments were obtained.
Conclusions:
- Formaldehyde is an effective agent for preserving alphavirus structure.
- The described methods facilitate biochemical, electron microscopic, and serological analyses of alphaviruses.