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Updated: Aug 7, 2026

A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
Published on: January 17, 2018
Total-field electron-microscopic examination of thin tissue slices via surface-embedding and multiple-mesa technics
This study introduces two novel techniques for total-field electron microscopy of thick tissue slices. These methods enable detailed examination of the entire cross-section of specimens up to 200 micrometers thick.
Area of Science:
- Electron Microscopy
- Materials Science
Background:
- Standard electron microscopy is limited to very thin samples.
- Examining thicker biological or material samples requires specialized techniques.
Purpose of the Study:
- To present a combined method for total-field electron microscopy of thicker tissue slices.
- To enable detailed cross-sectional analysis of specimens up to 200 micrometers.
Main Methods:
- Surface-embedding: Ensures the tissue slice remains planar during embedding for complete cross-section analysis.
- Multiple-mesa technic: A trimming method to create multiple examination targets on a single field.
Main Results:
- The described techniques allow for total-field electron microscopic examination of tissue slices.
- Successful application to samples with thicknesses ranging from 50 to 200 micrometers.
Conclusions:
- Combining surface-embedding and multiple-mesa techniques provides a robust method for analyzing thicker samples.
- These techniques enhance the capability of electron microscopy for detailed structural investigation.
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