An assessment of the Corning electrophoretic procedure for high density lipoprotein cholesterol estimation

The electrophoretic procedure for high density lipoprotein cholesterol determination recently introduced by Corning Medical has been assessed. The results corrrelated well with those obtained by phosphotungstate/Mg precipitation and ultracentrifuge procedures, but the between-batch CV was 6% in the clinically important range below 1 mmol/l. The densitometer, which must be carefully aligned before use for this analysis, seemed sensitive to variations in the plates and patterns produced. The technique required that great care, especially with timing, and appeared expensive.