Kinetics of human chromosome loss from 3T3-human hybrid cells

Human chromosomes were lost from populations of 3T3-HeLa and 3T3-diploid human fibroblast (HF) hybrids with similar first-order kinetics. Whereas loss began immediately in 3T3-HF hybrids, there was a lag of 5-10 cell divisions before chromosome loss began in 3T3-HeLa hybrids. Human chromosome loss was not affected by aminopterin selection, the use of polyethylene glycol rather than Sendai virus as fusagen, or by the presence of one or two 3T3 genomes. However, when cell division was retarded by growing 3T3-HF hybrids in low serum or at low temperatures, fewer human chromosomes were lost. This suggests that cell cycle traverse is important in chromosome loss. The distribution of human chromosomes among hybrid metaphases indicated that gradual chromosome loss occurred in all hybrids rather than extensive loss from a portion of the hybrids. During the period of chromosome loss, increased numbers of individual asynchronously condensed human chromosomes were randomly distributed among hybrid metaphases.