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In vitro granulocyte function as affected by filter loading time and tapping during filtration leukapheresis
Abstract:
Granulocytes (PMNs) were harvested from 16 normal subjects by filtration leukapheresis (FL) in which the filter loading time (FLT) ranged between 30 min and 4 h, and the rate of filter perfusion was 60 ml whole blood x min-1. The function of PMNs eluted from filters with or without the aid of manual tapping (MT) at each of the various FLTs was assessed in vitro by tests of chemotaxis, phagocytosis and bactericidal capacity. The results indicated that the function of PMNs in vitro was compromised by eluting filters with MT, and this appeared to be independent of the FLT. PMNs collected without MT were nearly normal relative to the control regardless of the FLT.
Insights
Manual tapping during filtration leukapheresis compromises polymorphonuclear (PMN) function. PMNs harvested without manual tapping maintain normal function, irrespective of filter loading time, indicating a gentler collection method is preferable.
Area of Science:
- Hematology
- Immunology
- Cellular Biology
Background:
- Polymorphonuclear cells (PMNs) are crucial for immune response.
- Filtration leukapheresis (FL) is a method for collecting PMNs.
- The impact of collection methods on PMN function requires further investigation.
Purpose of the Study:
- To evaluate the effect of manual tapping (MT) during FL on PMN function.
- To determine if filter loading time (FLT) influences PMN function after collection.
Main Methods:
- Harvesting PMNs from 16 healthy subjects using FL with varying FLTs (30 min to 4 h).
- Assessing PMN function in vitro using chemotaxis, phagocytosis, and bactericidal assays.
- Comparing PMNs collected with and without MT.
Main Results:
- Manual tapping during PMN elution significantly compromised PMN function in vitro.
- Compromised function was observed regardless of the filter loading time.
- PMNs collected without MT exhibited near-normal function compared to controls.
Conclusions:
- Manual tapping during filtration leukapheresis negatively impacts PMN function.
- Collecting PMNs without manual tapping preserves their functional integrity.
- Optimizing leukapheresis techniques is essential for obtaining functional immune cells.