[Infectivity of the DNA of PM-2 phage]

|January 1, 1980

Abstract:

The infectivity of PM-2 phage free DNA was studied versus competent cells of marine bacteria Pseudomonas Bal-31 depending on the time of incubation with the bacterial cells, on the DNA and calcium concentration in the solution. Optimal conditions for the infection are the following: DNA concentration about 3 microgram/ml, CaCl2 concentration 0.1 M, incubation time 20 min.

Insights

This study investigated the infectivity of PM-2 phage DNA in marine bacteria Pseudomonas Bal-31. Optimal conditions for high infectivity were identified, including specific DNA, calcium chloride, and incubation time parameters.

Area of Science:

Microbiology
Molecular Biology
Bacteriology

Context:

Marine bacteria, specifically Pseudomonas Bal-31, are crucial in oceanic ecosystems.
Bacteriophages, like PM-2, play a significant role in regulating bacterial populations.
Understanding phage-host interactions is vital for microbial ecology and biotechnology.

Purpose:

To determine the optimal conditions for the infectivity of PM-2 phage free DNA in competent Pseudomonas Bal-31 cells.
To investigate the influence of incubation time, DNA concentration, and calcium chloride concentration on phage DNA infection efficiency.

Summary:

The study optimized the infectivity of PM-2 phage DNA with marine bacteria Pseudomonas Bal-31.
Optimal conditions were found to be approximately 3 microgram/ml DNA concentration, 0.1 M calcium chloride (CaCl2) concentration, and a 20-minute incubation period.
These findings provide a quantitative basis for maximizing phage DNA transformation in this marine bacterial species.

Impact:

Establishes precise parameters for efficient genetic transformation of Pseudomonas Bal-31 using phage DNA.
Contributes to the understanding of phage-mediated gene transfer in marine environments.
Potential applications in marine biotechnology and genetic engineering of marine bacteria.

Read full analysis

Related Experiment Videos

[Infectivity of the DNA of PM-2 phage]

Insights

Related Concept Videos

[Use of U3 promotor from the LTR region of bovine leukemia virus for expressing genes for antiviral antisense RNAs in cell cultures].

[Study of conditions for cleaving bovine leukemia virus RNA in vitro using ribozymes directed against long terminal repeats].

[Genetic engineering in the bacterial synthesis of somatostatin].

[Synthesis of somatostatin in Escherichia coli cells: isolation and characteristics].

[Analysis of RAT2 cells, transformed by the CELO (FAV1) virus, using the polymerase chain reaction].

[Suppression of replication of swine parvoviral antisense RNA against the NS PPV gene in swine thyroid gland cells].

[Molecular characterization of a rare rotavirus (Sedoreoviridae: Rotavirus) strain genotype G2P[9] isolated from a child with acute gastroenteritis].

[Genetic characteristics of adenoviruses (Adenoviridae: Mastadenovirus) circulating among military personnel with respiratory infections during the 2023-2024 season in the Sverdlovsk Region].

[Test systems for the laboratory detection and differentiation of Powassan virus from tick-borne encephalitis virus (Flaviviridae: Orthoflavivirus) using RT-PCR and RT-qPCR].

[Rapid differentiation of genotype I and new recombinant variant of African swine fever virus (Asfarviridae: Asfivirus) using real-time PCR].

[Influenza virus infection affects the efficacy of the treatment of dyslipidemia in mice with a polyisoprenoid-based drug].

[Serological tests based on viral glycoproteins for detecting neutralizing antibodies to measles, mumps and rubella viruses].

Related Experiment Videos

[Infectivity of the DNA of PM-2 phage]

Abstract:

Insights

Related Concept Videos

Related Articles