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Enterotoxigenicity of aeromonas strains in suckling mice
Abstract:
The enterotoxigenicity of 170 Aeromonas strains isolated from different sources (food poisoning, random food sampling, water, faeces) was examined by the suckling mouse test. The strains were grown on Syncaye culture medium covered with sterilized membrane for Kiil-kidney. The culture supernatants were inoculated orally. Ileal loop dilatation was compared to that produced by the international standard enterotoxic Escherichia coli B7A (O148 : H28) and B2C (O6 : H16) strains. Of the 87 Aeromonas hydrophila strains 69, of the 76 Aeromonas punctate subsp. caviae strains 9, the 6 Aeromonas punctata subsp. punctata strains 5, and 1 Aeromonas salmonicida subsp. achromogenes gave a positive reaction in the test.
Insights
This study investigated Aeromonas strains for enterotoxigenicity using the suckling mouse test. Many Aeromonas hydrophila strains showed enterotoxigenic potential, indicating a risk in food and water sources.
Area of Science:
- Microbiology
- Food Safety
- Veterinary Science
Background:
- Aeromonas species are ubiquitous in aquatic environments and can be associated with foodborne illnesses.
- Assessing the enterotoxigenic potential of Aeromonas is crucial for public health and food safety.
- Previous studies have indicated varying levels of pathogenicity among different Aeromonas strains.
Purpose of the Study:
- To determine the enterotoxigenicity of 170 Aeromonas strains isolated from diverse sources.
- To compare the enterotoxigenic activity of these strains with standard enterotoxigenic Escherichia coli strains.
- To identify which Aeromonas species and subspecies exhibit enterotoxic properties.
Main Methods:
- Isolation of 170 Aeromonas strains from food poisoning cases, food samples, water, and faeces.
- Cultivation of strains on Syncaye medium with sterilized membranes for supernatant collection.
- Oral inoculation of suckling mice with culture supernatants.
- Assessment of ileal loop dilatation as an indicator of enterotoxicity, compared to Escherichia coli standards.
Main Results:
- 69 out of 87 Aeromonas hydrophila strains tested positive for enterotoxigenicity.
- 9 out of 76 Aeromonas punctata subsp. caviae strains showed a positive reaction.
- 5 out of 6 Aeromonas punctata subsp. punctata strains were enterotoxigenic.
- 1 Aeromonas salmonicida subsp. achromogenes strain also tested positive.
Conclusions:
- Aeromonas hydrophila demonstrates significant enterotoxigenic potential, posing a risk in food and water.
- Other Aeromonas subspecies, including Aeromonas punctata subsp. caviae and Aeromonas punctata subsp. punctata, also possess enterotoxic capabilities.
- The findings highlight the importance of monitoring Aeromonas contamination in food and water supplies to prevent public health issues.