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A simple method for the recovery of active oligomeric enzyme from its diluted solutions
Journal of Biochemical and Biophysical Methods
|April 1, 1980
Abstract:
A method for the recovery of active enzyme from its diluted solutions is described. It includes batch sorption on DEAE-Sephadex A-50 which follows elution or chromatography of an enzyme on a DEAE-Sephasex A-50 column. All purified aminoacyl-tRNA synthetases retain high activity without degradation of the protein. The method is very fast and convenient.