High-performance liquid chromatographic analysis of testosterone esters in oily solution

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Testosterone esters are widely used in hormone replacement therapy.
• Accurate quantification of testosterone esters in oily solutions is crucial for pharmaceutical quality control.

Purpose of the Study:

• To develop and validate a high-performance liquid chromatography (HPLC) method for analyzing testosterone esters in oily formulations.

Main Methods:

• Utilized a microparticulate silica column with a bonded hydrocarbon phase.
• Employed a mobile phase consisting of methanol-tetrahydrofuran-water (57:11:32 v/v/v).
• Detection was performed at a wavelength of 240 nm.

Main Results:

• The HPLC method demonstrated effective separation and analysis of testosterone esters in oily solutions.
• The established procedure provides a reliable means for quantifying these compounds.

Conclusions:

• High-performance liquid chromatography offers a robust analytical solution for testosterone esters in oily matrices.
• This method is suitable for quality control and formulation analysis in pharmaceutical settings.