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Updated: Aug 2, 2026
Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
Published on: November 20, 2013
Microanalysis of calcium in nerve
Abstract:
Free calcium in the range of 20--60 nM can be analyzed in single nerve fibers of the squid. Analytical methods used where: (1) the photoprotein aequorin, and (2) Arsenazo III, a dye which undergoes a spectral transformation when it interacts with Ca. The free calcium concentration of fresh squid axoplasm is 20--50 nM. Most cell calcium is buffered by organelles; only about 0.1% is ionized. Smooth endoplasmic reticulum appears to be a significant calcium buffer, and also, in some cases, the mitochondria located immediately adjacent to the membrane.
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