Paper chromatographic systems for the separation of aldosterone, 18-hydroxycorticosterone, 18-hydroxydeoxycorticosterone, corticosterone and deoxycorticosterone
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Updated: May 1, 2026

Extraction and Analysis of Cortisol from Human and Monkey Hair
Published on: January 24, 2014
Abstract:
Regulation of aldosterone (Aldo) biosynthesis is still open to controversy. The direct sites of action of the known stimulants (e.g., potassium, angiotensin II, adrenocorticotropin) on the different enzymes involved in the biochemical pathway of Aldo are not yet clearly defined. We have developed a radioimmunoassay for the determination of Aldo, 18-hydroxycorticosterone (18OH-B), 18-hydroxydeoxycorticosterone (18OH-DOC), corticosterone (B) and deoxycorticosterone (DOC) in plasma. The aim was to evaluate a single chromatographic system for the separation of these five mineralocorticoids before radioimmunoassay.
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