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Air bubbles and temperature effect on blood gas analysis

G Madiedo, R Sciacca, L Hause

    Journal of Clinical Pathology
    |September 1, 1980
    PubMed
    Summary

    Air bubbles in blood gas samples significantly elevate PO2 levels. Anaerobic blood samples stored at room temperature are reliable for analysis within 20 minutes.

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    Area of Science:

    • Clinical Chemistry
    • Medical Laboratory Science
    • Respiratory Physiology

    Background:

    • Accurate blood gas analysis is critical for patient diagnosis and management.
    • Pre-analytical variables like sample handling and storage can significantly impact results.
    • Understanding these variables is essential for reliable point-of-care testing and laboratory diagnostics.

    Purpose of the Study:

    • To investigate the impact of temperature, storage duration, and air bubbles on blood gas analysis.
    • To determine acceptable pre-analytical conditions for maintaining sample integrity.
    • To provide evidence-based guidelines for blood gas sample handling.

    Main Methods:

    • Blood samples were analyzed for blood gases under varying conditions.
    • Controlled experiments were conducted to assess the effect of air bubble contamination (10% proportion).
    • Storage at room temperature (25°C) was evaluated for anaerobic samples over time.

    Main Results:

    • A 10% proportion of air bubbles in samples caused a significant elevation in partial pressure of oxygen (PO2).
    • Storage of anaerobic blood samples at room temperature (25°C) yielded acceptable results when analyzed within 20 minutes.
    • Temperature and storage time effects were quantified for PO2, PCO2, and pH.

    Conclusions:

    • Air bubbles are a critical pre-analytical error source in blood gas analysis, particularly affecting PO2.
    • Short-term storage (within 20 minutes) of anaerobic blood samples at room temperature is permissible.
    • Strict adherence to proper sample collection and handling techniques is vital for accurate blood gas measurements.

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