Related Experiment Video
Updated: Jul 16, 2026

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
Published on: March 26, 2012
Early capping of transcripts from the adenovirus major late transcription unit
Abstract:
The various events which result in modification of a primary RNA transcipt may have a role in choosing transcripts which are to be processed into mRNA. If there is a stepwise and interdependent nature to each of the modifications then it is important to place the various steps in temporal order. We show here that the formation of a capped 5' terminus seems to be a very early event for adenovirus type 2 (Ad-2) nuclear RNA that is initiated at the major late Ad-2 promoter. There is an equally rapid entry of 3H-adenosine into the cap and the first dozen or so adenylate residues in the RNA chain. Taken together with evidence on general mRNA metabolism in Chinese hamster cells, it seems unlikely that capping has any differential role in successful RNA processing rather is an automatic event for all RNA polymerase II products.
More Related Videos
Related Concept Videos
pre-mRNA Processing
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Leaky Scanning
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability
Bacterial Transcription
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Pre-mRNA Processing: Modification of pre-mRNA Ends
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...

