The distribution of membrane-glycogen complexes in the orbital surface layer of rabbit superior rectus

The distribution of membrane--glycogen complexes along the length of individual muscle fibers was compared among three fiber populations in the orbital surface layer of rabbit superior rectus. These three populations were (a) 61 singly innervated fibers (SIFs), (b) 10 multiply innervated fibers of relatively constant 10 micrometer diameter (10 micrometer MIFs), and (c) 22 multiply innervated fibers which are of about 5 micrometer diameter toward the middle of their length and of about 15 micrometer diameter toward their proximal and distal segments (5--15 micrometer MIFs). The orbital surface layer was sampled by electron microscopy at 68 sequential locations. Membrane--glycogen complexes were not seen in any of the 1738 samples of the SIFs. In the MIFs, such complexes were observed in 14% of the 1541 samples. However, both the 10 micrometer MIFs and 5--15 micrometer MIFs displayed a preferential concentration of membrane--glycogen complexes toward their distal fiber portions, and such complexes were seen in about 50% of the MIF samples near the beginning of the muscle's distal third. In the distal portion of 5--15 micrometer MIFs, there was a direct relationship between their increasing fiber diameter and their increasing frequency of occurrence of membrane--glycogen complexes.