[Properties of the RNA of the new Okhotsk orbivirus]

Electron microscopic examination of Okhotsky virus RNA showed it to have double-stranded fragmented structure. Investigation of the kinetics of virus-specific RNA synthesis in chick embryo fibroblast cell cultures inoculated at a high multiplicity of infection demonstrated that the synthesis of virus-specific RNAs of Okhotsky virus reached maximum by 7 hours postinfection. A decrease in the multiplicity of infection slowed down the kinetics of virus-specific RNAs synthesis. The sedimentation analysis in sucrose density gradient showed the virus-specific RNAs of Okhotsky virus to sediment with a peak in 14.5 S band.