[Effects of uncouplers on the phospholipid composition of mitochondria]

A decrease in the level of lysocardiolipin and lysophosphatidylethanolamine in mitochondria induced by chlorophenacyl and 2,4-dinitrophenol was demonstrated. The effects of the uncouplers were especially well-pronounced under conditions of mitochondrial swelling. It was found that the phospholipase inhibitor nupercain completely reverses the effects. The data obtained suggest that 2,4-dinitrophenol and alkylating compounds increase the phospholipase activity of mitochondria under given experimental conditions.