Heterochromatic additivity and the CFF response
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Updated: Nov 6, 2026

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Published on: January 30, 2018
Abstract:
The validity of the heterochromatic additivity principle (Abney's Law) was examined using bichromatic combinations of test wavelengths adjusted to equal luminance by a CFF technique. Additivity failures qualitatively similar but smaller than those resulting from foveal threshold, incremental threshold, or direct brightness matching techniques were evident for a number of bichromatic combinations. These results suggest that the CFF response is influenced, to a small degree, by the activity of opponent-color channels.
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