Functional platelets after storage in vitro for 15--21 days

Area of Science:

• Hematology
• Biochemistry
• Cell Biology

Background:

• Platelets are crucial for hemostasis but have limited in vitro storage viability (72-96 hours).
• Current storage methods result in loss of platelet function, necessitating routine discarding after three days.

Purpose of the Study:

• To evaluate the effect of increased pH in anticoagulant on platelet functional viability during room temperature storage.
• To determine the long-term preservation potential of platelets using this modified collection method.

Main Methods:

• Collected blood using anticoagulant with elevated pH to prepare platelet-rich plasma (C-PRP).
• Stored C-PRP samples at room temperature for up to 21 days.
• Assessed platelet count, responsiveness to aggregating agents (ADP, SA), serotonin levels, arachidonic acid metabolism, adenine nucleotide levels, and physical morphology.

Main Results:

• Platelet counts decreased, but a significant proportion remained viable for 21 days.
• Platelets retained responsiveness to ADP and SA through Day 21.
• Biochemical markers (serotonin, thromboxane B2 conversion) and adenine nucleotide metabolism remained favorable.
• A portion of stored platelets maintained discoid shape and cellular structures.

Conclusions:

• Elevated pH during blood collection and C-PRP preparation enhances long-term platelet preservation in vitro.
• This method supports sustained platelet function and viability beyond conventional storage limits.
• Findings suggest a potential improvement for platelet storage protocols in blood banking.