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[Triiodothyronine radioimmunoassay. Comparison of the different factors between two technics (author's transl)]
Triiodothyronine concentration was determined in human serum and in thyroid extracts by two radioimmunoassay technics. Both antibodies, radioactive T3, stable T3, protein-binding-hormone inhibitors, methods used to separate the free from the bound fractions were different. Each factor from a method was substituted to the homologue of the other method. The results occasionally showed a variation of 32 p. cent in the first method and 50 p. cent in the second one. However the results gave a correct interpretation of the triiodothyronine values measured in human serum and in thyroid extracts.
Triiodothyronine concentration was determined in human serum and in thyroid extracts by two radioimmunoassay technics. Both antibodies, radioactive T3, stable T3, protein-binding-hormone inhibitors, methods used to separate the free from the bound fractions were different. Each factor from a method was substituted to the homologue of the other method. The results occasionally showed a variation of 32 p. cent in the first method and 50 p. cent in the second one. However the results gave a correct interpretation of the triiodothyronine values measured in human serum and in thyroid extracts.