A discrete multichannel procedure for the determination of acid phosphatase activities in serum

The purpose of this study was to increase the capacity of the laboratory to perform analyses for the acid phosphatase activities by adapting the previous manual method to a discrete multichannel, computer controlled photometer. The modified system was far superior to the manual method, showing an improvement in the accuracy and precision of the method as well as a three times higher efficiency of the method for analyses. The reference values for serum acid phosphatase and its tartrate stable fraction were determined from apparently healthy women and men. The clinical significance of the method was discussed on the basis of the year's experience.